GN Broth (Hajna) is used for the selective enrichment of Gram-negative organisms in a laboratory setting. GN Broth (Hajna) is not intended for use in the diagnosis of disease or other conditions in humans. Hajna formulated Gram Negative (GN) Broth as an enrichment medium for enteric Gram-negative bacilli, especially Salmonella spp. and Shigella spp. Croft and Miller demonstrated improved recovery of Shigella spp. using GN Broth enrichment compared to direct inoculation of agar. Taylor and Schelhart reported improved recovery of Salmonella spp. and Shigella spp. when using GN Broth enrichment compared to direct inoculation of media. Taylor and Schelhart found GN Broth to be superior to selenite enrichment medium for recovering Shigella spp. This broth is used as a nonselective enrichment to recover Salmonella spp. and Shigella spp. from food.
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