The EBX-041 is a test using reverse-transcription and real-time amplification of viral RNA of SARS-CoV-2 based on amplification of 3 genes (RdRp Gene Target 1, RdRp Gene Target 2 and N Gene target 3). If at least one RdRp target is positive, a SARS-CoV-2 positive diagnostic can be called (see Data analysis and Interpretation).
The kit contains one oligomix to detect the 3 targets, as well as an encapsidated control of RNA extraction and RT-PCR inhibition. The test is performed from extracted RNA from a sample, using one RT-PCR reaction in a single distinct well/tube.
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